16 x 16 pixels – 32bit (24bit colour, 8bit transparency).32 x 32 pixels – 32bit (24bit colour, 8bit transparency).48 x 48 pixels – 32bit (24bit colour, 8bit transparency).256 x 256 pixels – 32bit (24bit colour, 8bit transparency).If you want to create an Windows 7 Compliant ‘Application Icon’, to be used as a short-cut, a file type, or embedded in an executable file, it must contain the minimum following icon sizes: Standard Windows Icon Size for ICO format Standard Windows Icon Sizes shown in Axialis Icon Workshop This is in some way brought about by the flexibility of the ICO format and its ability to ‘contain’ many image sizes and colour depths but is also not helped by Microsoft’s own quite poor documentation on Windows Icon Size, see here. There is sometimes a little confusion over the different sizes required to create a Windows 7 Application Icon file. A Quick & Easy guide to Microsoft Windows Icon Size